Sign up now!
Don't show this again
Download the report!Continue to Site >
or wait 7 secs

Thank you for confirming your subscription!

(And remember, if ever you want to change your email preferences or unsubscribe, just click on the links at the bottom of any email.)

We’re glad you’re enjoying Pig Health Today.
Access is free but you’ll need to register to view more content.
Already registered? Sign In
Tap to download the app


Collect articles and features into your own report to read later, print or share with others

Create a New Report


Read Later

Create a new report

Report title (required) Brief description (optional)
follow us

You must be logged in to edit your profile.

Favorites Read Later My Reports PHT Special Reports
Pig Health Today is equipped with some amazing (and free) tools for organizing and sharing content, as well as creating your own magazines and special reports. To access them, please register today.
Sponsored by Zoetis

Pig Health Today | Sponsored by Zoetis

Featured Video Play Icon

Processing fluids helping to fine tune diagnostics in swine herds thought to be PRRS-negative

Collecting aggregate samples of processing fluids during tail docking and castration is proving to be an excellent way to monitor herds for porcine reproductive and respiratory disease (PRRS) virus, Will Lopez, DVM, Iowa State University, told Pig Health Today.

Lopez said this method is yielding “amazing” results and can detect the presence of PRRS virus early and save producers time and money.

The procedure simply involves placing all tails and testicles removed during processing into a bucket lined with a plastic bag and cheesecloth, which are secured around the top of the bucket with a large rubber band. Fluids from the tissues drain through the cheesecloth into the plastic bag. After collection, a small hole is made into the bottom of the plastic bag so fluids can be funneled into testing vials and sent to a lab for testing.

Better sensitivity

Results of a study conducted by Lopez and colleagues showed processing-fluid testing detected PRRSV RNA by rRT-PCR (real-time reverse transcription-polymerase chain reaction) with far greater sensitivity compared to results with matched individual pig blood sera or tail blood swabs pooled into groups of five.

The procedure is better than oral-fluid sampling using ropes in some situations, Lopez noted, because it can be difficult to get very young pigs to chew on ropes.

So far, the highest sensitivity with the processing-fluid method has been found when fluids from an entire day of collection are tested by PCR, he said.

It’s not uncommon, Lopez explained, for re-breaks of PRRS to occur on farms thought to be negative or stable for PRRS with the same strain of virus detected before a control or elimination plan was implemented. This suggests the virus was never gone and that it can circulate at a very low prevalence for an undetermined period of time.

Test more pigs, more often

“That’s kind of the motivation we want. We need to test more pigs more frequently in order to really increase our probability of detection of the virus at near-zero prevalence,” he said. With processing fluids, the sample size can be increased as well as the frequency of testing. Less labor time is required for testing. It also costs less.

There are limitations, however. This method does not reveal how many piglets are positive out of the sample, but Lopez and colleagues are working with statistical processes that might help determine how many piglets are positive or negative in a given sample. The other downside is the possibility of environmental contamination of samples.


Posted on October 10, 2018

tags: , ,
  • Common mistakes to avoid during PRRS elimination

    Eliminating porcine reproductive and respiratory syndrome (PRRS) virus from a breeding herd is not a simple task, and no herd acts exactly like the next, according to Brad Leuwerke, DVM, with Swine Vet Center, St. Peter, Minnesota.

  • African swine fever, PRRS among hot topics at 2021 Leman Conference

    Organizers are excited to hold the 2021 Allen D. Leman Swine Conference in-person as well as virtually, on Sept. 18-21 in St. Paul, Minnesota.

  • Processing fluids provide an option to monitor PCV2 and PCVAD

    Effective PCV2 control relies on vaccination of healthy pigs before they become infected. This goal cannot be accomplished in unstable herds whose sows give birth to viremic pigs.

  • Can the processing-fluid toolbox expand beyond PRRS?

    Piglet processing fluids have been shown to be a practical, time-efficient and affordable diagnostic tool for PRRS, and some indications suggest that PCV2 offers promise as well.

You must be logged in to edit your profile.

Share It
US producers and veterinarians have seen an influx of different types of influenza viruses in the last 10 to 15 years, and that is a major reason why influenza is more difficult to control.

Click an icon to share this information with your industry contacts.
Google Translate is provided on this website as a reference tool. However, Poultry Health Today and its sponsor and affiliates do not guarantee in any way the accuracy of the translated content and are not responsible for any event resulting from the use of the translation provided by Google. By choosing a language other than English from the Google Translate menu, the user agrees to withhold all liability and/or damage that may occur to the user by depending on or using the translation by Google.